FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673221
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72210.12728219119262232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19250.014.5172741
GTATAGA23550.014.3570751
TATACTG27050.014.0292115
CTAGACT9100.013.6479974
GTCCTAC7350.013.1432111
CTAATAC21150.012.886193
TAATACT33750.012.4707874
CCTACAC9800.012.3211093
GTATATA35650.011.5164391
CTATACT20650.011.360484
TAGTACT15500.011.3513194
CTCCTAG7700.011.20161
TCTAGAC15150.011.1581263
TAGGACA12750.011.093834
TCTATAC21550.011.0461173
GTATAAT30650.011.0312921
TACACAG17300.010.9679245
TATAATA40300.010.9575322
TAGACAG19850.010.9493395
CTATGCA18950.010.9218519
Produced by FastQC (version 0.11.2)