FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611264
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48540.13441277070853863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8500.017.8584525
ACGCGCA5300.015.6264339
TAGGCCG2654.1802195E-815.6223155
GACGCGC4950.015.3370538
CGCGCAT4600.015.00363640
GTATTAG15250.014.9312491
TATTAGG12550.014.5694162
TATTATA22000.014.2701572
TAGTCAG7500.014.2571349
CGCACCT5350.014.18504766
TGACGCG5900.014.03730137
GGTAGTC8700.013.8776757
GTATCCT15250.013.8000941
GTATTAT23700.013.3924961
ACCCTAG5950.012.756391
GTATATA21400.012.5748531
TAGGAGT10500.012.1600934
TCCTTGC22600.012.0574778
GTATATG18000.011.8834141
CCTATAC13700.011.8353553
Produced by FastQC (version 0.11.2)