FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272103
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28140.12385001912325276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG13050.018.7685875
GTACTAG5650.015.8758631
GTCTTAG4400.014.8974981
GTATTAG7600.014.5260931
CCCTATA4801.8189894E-1213.6551382
ATATACT16650.013.4673734
TCTATAC11050.013.4242473
CCTATAC6700.013.386973
GTATCCT9550.013.3662871
TATATAC15300.013.3028393
GTATAGG7300.013.2326741
GTATATG10500.013.1426551
TATTATA15450.012.9504022
ACCTAGG2457.7307384E-512.6732751
ATTATAC12150.012.492813
GTATTAA10250.012.4534671
ATACTGT15000.012.4189896
TCCTAAG6950.012.4090612
TTATACT12350.012.01116854
CTAGACT4903.6925485E-1011.9684134
Produced by FastQC (version 0.11.2)