FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529746
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41240.11683560233512554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT5600.017.86569666
GTATTAG13500.016.3559071
CTAATAC14700.016.193773
CTTACAC10800.015.6526723
TCTATAC16350.014.981553
TCCGCAC7600.014.98016264
CTATACT14550.014.7007234
GTATATA22000.014.5844051
TAGACCG9600.014.3749035
CGCGACC1450.0052169714.2761719
TATACTG17950.014.2227455
CCGCACC6850.014.10189465
TATACAG18250.013.9889455
TAGGACT6850.013.0946164
CCTATAC12500.012.9719123
CTATACA16950.012.822744
TATACCG10550.012.75346855
TATAAGG12200.012.7255042
CCTAATA11200.012.6295582
TATATAG16150.012.603635
Produced by FastQC (version 0.11.2)