FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520217
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66790.1209916204381096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11150.015.7799455
GTATTAG22800.015.7372321
CTAATAC21850.015.3157533
GTATAGA25300.014.4549041
GTATATA37450.014.2793931
ACCGTGC8700.014.2732418
TATACTG29700.014.1714265
CCTAATA22900.014.1616672
CGCACCT9850.014.00948466
TCTATAC23350.013.8886183
CCGTGCG3557.357812E-913.6028899
TATACAG24950.013.13600355
CTAGGAC10350.012.99996953
TCCGCAC10650.012.95748164
TATTATA39150.012.8659872
GTACTAG15150.012.7527921
TACACCG12000.012.3622475
CTATACT22400.012.3211764
TATACCG15350.012.13655955
TAATACT35450.012.0674524
Produced by FastQC (version 0.11.2)