FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159589
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33630.15572407527543436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7500.017.479161
GACCGCG2057.7712866E-716.8272917
TATTAGG7350.016.4277822
GTATAGA9800.015.8410761
CTAATAC8050.015.4281873
TAGGTAG5650.015.2647535
TATACTG13750.015.05382255
CTTACAC6550.014.2210213
TTAGGTA8600.014.0403494
CTCCTAG3708.749339E-1013.9858151
CTATACT10400.013.9323464
GGCACGC3001.9037543E-713.80221536
CCTATAC7750.013.7996583
GTCCTAG3001.908611E-713.7993371
TAGACTG4801.8189894E-1213.6555935
TATACCG6700.013.3874175
ATTAGGT8250.013.3814873
TATACGG7250.013.3234975
CTAGACC6500.013.2689024
GTCCTAA5300.013.0182441
Produced by FastQC (version 0.11.2)