FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6926941
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85470.12338779845244821No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29600.019.231551
TAGGTAG15800.017.2496195
TCCTTGC43150.014.4699318
TAGTCAG15450.013.6196799
CTCCTAG12050.013.1702121
TATTAGG26850.013.1056652
CCTTGCA30850.012.7472129
TATACGG25200.012.731865
GCGCACT11800.012.57084711
GGTAGTC17100.012.5075917
GTATCCT31050.012.2223051
TTAGGTA27250.012.1539464
CCCTTAG23200.011.8966311
CTAATAC29000.011.8961163
TAATACT46350.011.6859084
CAGCGCA16550.011.255419
GCGACGT14450.011.222095544
TCCTAAG18500.011.1887792
CCGTGCG4351.2482815E-711.1020799
GTATAGA28250.010.9912241
Produced by FastQC (version 0.11.2)