FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031142
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87000.10832830499074726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG14650.014.1293215
CTCGGAG16850.013.7168017
GCTCGGA17000.013.3936826
GATACTC65350.011.8766369
GTATTAT37850.011.75787451
GTATTAA26650.011.5212011
GTATAGA25000.011.4536281
TATACAG24900.011.0840465
GTATTAG21650.010.9950241
GCCGACG168000.010.98501969
TATTATA32800.010.9386692
TATAAGG22150.010.7468282
ATTATAC31950.010.6898593
CGATACT80650.010.6500469
CGGAGAT27600.010.6232659
TATTAGG22000.010.5064772
TTATACT29100.010.3142264
TCTATAC24850.010.2733723
CCTAATA22300.010.0557272
TCCTATA17300.09.9707752
Produced by FastQC (version 0.11.2)