FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472037
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57760.12915814426401215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT8450.014.69664866
CTAATAC19600.014.4326093
GTATTAG18750.014.1682221
TATACAG24200.013.9702015
GTCCTAG6050.013.6861661
CTAGGAC6050.013.6849413
TAGGTAG10350.013.6658125
TCCGCAC9250.013.42573664
CCGCACC9050.013.3409665
TAATACT30500.012.894424
GGCTCGG7650.012.1757095
CTCGGAG8600.012.0335927
GGTAGTC9500.011.98292357
GCCGACG29950.011.9776169
GCTCGGA8100.011.9249126
CTATACT20550.011.7512214
TCCTATA15600.011.7202892
GTATTAC20400.011.6693011
ACTATGC19550.011.64489658
GTACTAG13050.011.6323671
Produced by FastQC (version 0.11.2)