FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5363292
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71860.1339848734695034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13950.017.3119435
GTATTAG22000.015.368571
CTAATAC24000.014.2314773
TAGTCAG13100.014.219089
ACCGCGC4102.8376235E-1013.4614958
GTATATG31450.013.1640851
TAATACT39200.013.0255974
GTATATA53600.012.8734591
GGTAGTC14950.012.6913277
CGCACCT9300.012.61239966
TATTAGG23550.012.5989272
GTATAGA26500.012.2380451
TCCGCAC9900.012.19625464
CTATGCA21250.012.012189
GTCCTAG9500.011.9845131
TATACAG31400.011.7564485
ACTATGC23700.011.64390858
CGCGCAT5703.274181E-1111.50211540
TATACTG35350.011.4187465
TTAGGTA22700.011.3987544
Produced by FastQC (version 0.11.2)