FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736455
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44620.11941800449891675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18900.016.247231
GGCGTCG5800.015.46707232
TAGGTAG9350.015.1284455
TATACAG21500.014.1210255
GTGTACG6700.013.9040111
CGCGCGG3052.3380562E-713.57222754
GTATAGA16300.013.5470121
TCTATAC15800.013.5382473
TATACCG10250.013.1269195
TATTAGG15050.013.0667092
CGCGGGA3204.1909516E-712.93637656
TCCTAGG4852.3646862E-1112.8043822
TTAGGTA14650.012.716814
GTATATA32900.012.6893851
GTATTAT30250.012.6604381
GGTAGTC9700.012.4477057
TCCGCAC5850.012.38353864
GTATAGC12400.012.2428381
GGCGTCC7650.011.72604221
TAGTCAG9150.011.6868829
Produced by FastQC (version 0.11.2)