FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248148
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53610.1261961683067539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC18150.014.6378373
CGCACCT7200.013.89412466
GTATTAG18150.013.8779211
CCTTGCG12950.012.785779
TATACAG24550.012.6483365
CGCGCAT4501.2296368E-912.26833740
GACGCGC4256.903065E-912.17812738
ACGCGCA4551.4624675E-912.13351939
GTATAGA20550.012.0892371
GTATATA35750.011.9680611
TATACGG14150.011.9476375
CCGCACC8100.011.924458565
TATACCG12450.011.9163025
GTATTAT33650.011.7920941
GTCTAGA11500.011.7015921
TAGGTAG12400.011.686115
ACTATGC20150.011.6415218
GTATAGG12650.011.4561041
CCTACAC7550.011.4249923
GGTAGTC12100.011.4043617
Produced by FastQC (version 0.11.2)