FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821063
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58160.12063729513594823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG3351.0913936E-1116.4754529
GTATTAG18550.015.4368751
TCCTTGC28650.014.8098838
TAGGTAG10500.013.470925
CGCGCAT5500.013.17482840
CCTATAC18600.013.1688593
CCTTGCA22150.013.0818059
ACCCGCG5053.6379788E-1212.9814633
GTATCCT18950.012.9263051
CCTACAC9650.012.869983
CCCTTAG17700.012.4747531
CTATACT23600.012.2791984
GTATATG25600.012.1290781
GTCCTAG7150.012.06310751
CTAGACT10100.011.9549954
CTTAGAC15650.011.9037043
ACCGTGC6100.011.8757388
TCCTAAG14900.011.8087692
GTATAGA21750.011.7380971
CCTCCTA10650.011.6621371
Produced by FastQC (version 0.11.2)