FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867763
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46210.11947474547949292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16550.020.0121961
TAGGTAG9700.018.849165
TCCTTGC26400.015.2878818
TAGTCAG9050.014.4840229
CCTTGCG10800.014.0534539
GCTCGGA8200.013.462626
GGTAGTC11300.013.43248757
CTATACT17650.013.2908294
TCCTATA15400.013.2167522
TATTAGG17500.012.8135292
GGCTCGG7300.012.7593565
ACATAGG11550.012.2480931
CTCGGAG9050.012.1978597
ATAGGAG11050.012.17764233
CCTATAC15500.012.018493
TTAGGTA16700.011.7746024
GTATATA33150.011.6561741
AGGTAGT15400.011.6486636
TATACCG11550.011.6485125
CATAGGA12800.011.59125932
Produced by FastQC (version 0.11.2)