FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911395
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72040.14667930394521314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA53250.014.5127081
GTATTAG20700.013.83336451
TATACGG18150.012.3555255
TCTATAC23300.011.6972133
TATTAGG23600.011.6948222
TAGGTAG9750.011.6770695
GTATTAT44650.011.5129041
CTATACA24650.011.4765834
CTAGACT10000.011.3849094
CGCACCT8900.011.24091166
GCTTAGG9300.011.12735769
TATACTG31950.011.1222045
GTATAGA21750.010.9448521
TCCGCAC9150.010.93411664
GTGTAAG12550.010.7211391
CATATAC34300.010.6616133
TATACAG28800.010.6615925
CCGCACC9400.010.64309865
TCTAGAC13950.010.6342143
GGCTCGG6909.094947E-1210.5001315
Produced by FastQC (version 0.11.2)