FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170292
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63820.15303484743993945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18750.014.9051071
CTAGGAC5150.014.0678243
GTATAGA19100.013.3674841
CTCCTAG7150.012.5463871
GTATAGG12500.012.1449011
GGGCTCG7200.011.5001642
ACAGCGC7250.011.4193458
TAGGTAG10650.011.3380545
CTAGTAC9150.011.3113653
GTATATA45750.011.23691
CCGGCCC4955.513357E-911.15073713
AGTCCGG7450.011.11318610
TATATAC48150.011.1058113
GGTAGTC9350.011.0688797
GCCGACG25600.011.04908369
GTATTAA24200.010.9780881
GGCTCGG7600.010.8946313
GTATTAT35200.010.8800691
TAGACAG13150.010.7566795
GCTATAC11600.010.7067933
Produced by FastQC (version 0.11.2)