FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187669
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56310.1344662149754434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17700.015.7871911
TCCTTGC27500.014.8020458
GACGCGC4557.2759576E-1213.65014638
TCCTATA17050.013.3538812
CGCGGCA3651.0837539E-813.23224448
CTTAAGG9800.013.0244223
GGCTCGG6100.013.0072645
GTATCCT18500.012.6803251
TCGCGGC3551.0750773E-712.633247
ACGCGCA4953.274181E-1112.54740239
CGCGCAT5054.7293724E-1112.29908640
CTTACAC11850.012.2268253
CTTCCTA14600.012.0506491
GTATAGA18550.011.7162811
CCTTGCA19200.011.6784329
TAGGTAG10650.011.6611365
TAGACAG13150.011.5428995
CCTATAC15350.011.4615823
CCTACAC9350.011.4375473
TATACGG13050.011.3670025
Produced by FastQC (version 0.11.2)