FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821578
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73290.15200417788533133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG13300.017.6369869
TCCTTGC31800.016.2716358
GTATTAG21850.015.1574961
TAGGTAG11000.014.4265935
CCCTTGC13450.013.8496448
ACCCTTG17950.013.83721357
CCGCGCT4253.45608E-1113.80250136
GTATCCT19100.013.7273871
TCCTATA21800.013.2930912
TCCTAGG5750.013.1995162
ACCCGCG4254.984031E-1012.99072434
TATTAGG20250.012.9476732
CCTTGCA24350.012.8916819
CGCGCGG2952.37924E-612.86300154
CCGACGC4408.6583896E-1012.54382369
CCCGCGC4701.8371793E-1012.48111535
TCTATAC23150.012.0710813
ATCCTTG43450.011.6710027
ACCCTAG7700.011.6490441
TTAGACT17950.011.531494
Produced by FastQC (version 0.11.2)