FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304737
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55600.12916003927766087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG4400.018.03125454
CGCGGGA4100.017.66836456
TCCTTGC30100.016.3870268
CCTTGCG12900.013.9038199
AACGCGC5500.013.79766952
GTATCCT20100.013.5586721
CCTCCTA8450.013.0640961
GTATATG23800.013.0452271
GTATTAG16950.012.8220351
GCGCACT6300.012.59281711
GACGCGC4953.274181E-1112.54785638
CCTTGCA21600.012.2958199
CCGCGCG3651.547669E-712.2848839
TGACGCG5401.0913936E-1112.14135437
TCCTATA15400.012.0960632
GTATATA38300.011.8894371
GCGCGGG6700.011.84169655
CGATCTA19000.011.80063869
ACGCGCG5853.6379788E-1211.79274353
TCCTAAG10500.011.1701912
Produced by FastQC (version 0.11.2)