FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4577784
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64090.14000223689016345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19400.017.7849031
TCCTTGC30050.015.9577398
CCTTGCG13350.014.47067459
CCCCTAG4455.456968E-1213.9561531
TAGGTAG10900.013.6108645
CTAGACT6850.013.5993114
CCTAGAC9900.012.894533
GACGCGC4551.07320375E-1012.890841538
GTATAGA19900.012.8301541
CCTCCTA9450.012.7787821
GGCTCGG6500.012.7390453
TATTAGG17750.012.6342432
GTATAGG14550.012.5679971
GGTAGTC10650.012.309757
TAGACCG10400.012.27473455
ATCCTTG39900.012.1916217
GTATTAT33400.011.9829771
TATACTG28000.011.9524635
CTATACT19050.011.7723224
GGATCCT16750.011.741221
Produced by FastQC (version 0.11.2)