FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5340679
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80740.15117927888944457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG5200.024.54446654
CGCGGGA6100.020.35899756
AACGCGC7550.016.90479952
GTATCCT23200.016.6558441
ACGCGCG7750.016.023653
TCCTTGC40750.015.575048
ACGTCCC10450.015.5160645
CGCGCCG6550.015.27413715
CGCACCG7550.015.08205736
TACGTCC11900.015.0749684
CCTTGCA26850.014.9017199
CGTCCGG7200.014.85629732
CCGGAGC7700.014.78935940
ACGCGCA7000.014.78922139
GACGCGC6600.014.63928738
TCCTAGG6950.014.3958592
CCTTGCG17000.013.7969369
GCGAACC9050.013.72482623
GCGCGGG9600.013.65462655
GTATTAG20250.013.6301791
Produced by FastQC (version 0.11.2)