FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5552283
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83100.14966816352840806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT26050.018.938181
CGCGCGG5350.018.6995354
CGCGGGA5550.018.64741756
TCCTTGC37050.017.9696648
CCTTGCG17250.017.3972829
AACGCGC7250.015.22630652
CCTCCTA10650.014.9010941
ACGCGCG6600.014.63526353
CGTCCGG7100.014.09315832
CCTTGCA25900.013.4515669
GCGCACT8500.013.39238611
GTATTAG21250.013.312661
CTCCTAG8600.013.2380921
ATCCTTG49200.012.9715867
CCTATAC17850.012.9490743
TACTCCT24250.012.803745
TCCTAGG6500.012.7380782
CGCACCG7400.012.58950236
CGGCGCG5004.0017767E-1112.4215337
GTCCTAA11400.012.4076351
Produced by FastQC (version 0.11.2)