FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6283324
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111040.17672174791559372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT27050.022.447891
CGCACCG12650.021.82151636
CGCGCCG9850.021.71490315
CGGGCCG14350.019.23393822
CGTCCGG12700.019.01744332
ACGGGCC14900.018.7556621
GGCTCGC11250.018.70386511
CCGGAGC14300.018.57931740
GCTCGCG11800.018.41717112
TCGCGCC11250.018.39841714
GCGCACC15400.017.92453235
CACGGGC15700.017.58006520
GCGCCGA12250.017.1790716
CCGAGGG9300.016.6925164
CGCGCGG6900.016.49792354
CTCGCGC12350.016.48036213
TCACGGG17000.016.43878419
TCCTTGC38850.016.3373228
CCTCCTA9750.016.2773361
CCCAGGG12550.016.2182456
Produced by FastQC (version 0.11.2)