FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654766
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115430.6975608635903807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCC3250.025.4746464
GTATCCT6600.022.4759431
ACGTCCC3300.020.9078545
CCCCTGA3100.020.0310731
CCTTGCG5250.019.053719
TCCTTGC11250.019.0099098
CTTCCTA6100.018.662831
CCGAGGG3600.017.2463764
TGTGTAG6750.016.8610869
CGCGCGG1452.72518E-416.65368554
CGCGCCG3157.6397555E-1116.42859315
TGCCCGG1704.9603754E-516.23629823
GTATTAG5000.015.8690611
GGACTAA3950.015.71631269
TCCTCGC4400.015.6789958
GCCCGGA1350.003241205615.33520824
CCCGGAC1350.003241858715.33474425
GCCCCTA2252.0502885E-615.3324261
TAGGTAG3151.3587851E-915.3324265
GATTACG4950.015.3324261
Produced by FastQC (version 0.11.2)