FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811257
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88240.15184322428004818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC41800.018.4867578
GTATCCT27350.015.1382791
CCTTGCA29200.014.1767349
GTATTAG25950.013.6946971
TAGGTAG13400.012.8730195
ACCCTTG21000.012.813627
CCTTGCC22900.012.6538189
ATCCTTG55600.012.5955677
GGTAGTC13400.012.3575677
CGCGGGA4853.110472E-1012.09153156
CCTTGCG17200.012.033749
CGCGCGG4903.6925485E-1011.96804354
CCTCCTA12350.011.7336991
CTTCCTA24250.011.666881
GACGCGC5401.5097612E-1011.50177938
TCCGCAC10250.011.44301164
ACCCTAG9050.011.4373461
GTATATG35850.011.3565241
GCGACGT8900.011.24224744
GTCCTAC8600.011.2334241
Produced by FastQC (version 0.11.2)