FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988023
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95590.15963532538201675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT28750.019.4403341
TCCTTGC43450.016.83098
CGCGGGA5850.015.92215356
CGCGCGG5900.015.78669354
CGTCCGG6150.015.14736132
GTATTAG26100.014.9370381
CCTTGCA30500.014.8158129
TAGGTAG13250.014.5801645
CCTTGCG19350.013.191829
GCGAACC8900.013.18044223
CCCCTAG5004.0017767E-1112.4202141
GCGCGGG8900.012.40356755
GACGCGC6150.012.34373938
CCGTCCG7350.012.20510831
GTCCTAA13900.012.1620791
CGCGCAT6250.012.14644140
GTATGCT23650.011.9621511
GTATCCC15250.011.5379041
TTCCTTG71600.011.5149137
GGATCCT19600.011.4415241
Produced by FastQC (version 0.11.2)