FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605849
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58370.1618758855404095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTAG13700.022.6612969
TCTGTGT33150.018.73059869
CTCTGTG42200.018.71913369
GCTCGGA7950.016.9239736
CTGTGTA18800.016.514568
GGCTCGG7150.015.44026855
TACTCTG70700.014.198297569
CTCGGAG8400.013.9632477
GTATTAG9150.013.1975721
ACTCTGT72400.012.91199769
CTACTCT88500.012.66784569
ATCTACT131350.012.44832669
TCTACTC101050.012.25521169
GATCTAC153500.012.18017669
CTAGGGG2000.003100653212.0747723
CGATCTA183350.011.89046569
GTGCTAG7350.011.7354491
TAGGTAG6109.094947E-1211.3112615
CACTCCG4955.506081E-911.1514614
GTGTATA11850.010.77194816
Produced by FastQC (version 0.11.2)