FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307316
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47980.14507231846004434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG3000.020.69856654
TCCTTGC26100.020.2218198
GTATCCT14300.020.0252081
CCTTGCG11200.017.8632139
CGCGGGA3650.017.0125256
CCCTAGG3257.2759576E-1216.9844672
ACGCGCG4350.016.65401853
CCTTGCA16700.016.5243029
CCTAGGG3157.8216544E-1116.4281793
CCGGAGC5100.015.56127640
AACGCGC5050.015.02843552
GCTAGAC7150.014.95768453
CACGGGC6000.014.95054820
CGCACCG5150.014.74018836
GTATTAG13150.014.6925531
CCCCCTA3302.6229827E-914.63691
CGACGTA5000.014.49096945
TACGTCC5550.014.296744
AACTCCT18000.013.991125
CTTACAC8150.013.9689913
Produced by FastQC (version 0.11.2)