FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6974386
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95210.1365138092442833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25350.014.5629891
TCCTAGG11050.013.4255542
TCCTTGC38300.013.3297028
CTATACT27850.013.1309874
TATACGG23500.013.0658865
ACGCGCA7200.012.93882239
CCTATAC21700.012.8779023
CTCCTAG10100.012.639361
GACGCGC6900.012.50154638
CGCGCAT7850.012.30734340
TATACTG40000.012.1611845
TCCTAAG18050.012.0417442
CTAATAC29000.011.7776063
ATCCTAG13650.011.627061
CGCACCT10500.011.49952466
TTATACT43000.011.4731934
GTATAGA29000.011.4213651
CCTCCTA11800.011.4032161
CCTACAC13700.011.3321363
TATACAG35350.011.32101255
Produced by FastQC (version 0.11.2)