FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853256
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40520.14201319475013807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG9600.019.4023559
ACCCTAG3650.018.9076081
TAGGTAG6600.016.2069765
TCCTTGC21250.015.5835728
GTATCCT13850.014.2011831
CCCCTAA6000.013.8025541
ATCCTTG27650.013.7243057
CCCCTAT4951.8189894E-1213.2448751
GGCTCGG4457.2759576E-1113.1817525
GTATTAG10750.013.1605751
CCCTAGG2901.9570616E-613.0883992
CCTAGGG2952.3713656E-612.8663363
CTCGGAG6500.012.7376927
CCGGGCG1900.002113062212.7079759
TACTCCT14300.012.5473365
GGCCCCC2509.347013E-512.41881353
GTGTATG9750.012.3869071
GTCCTAA6750.012.2689381
GGATCCT8800.012.1556591
TCCTAAC9100.012.1339022
Produced by FastQC (version 0.11.2)