FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252995
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11750.017.0331634
GTATTAG21450.016.2475091
TAGGTAG11950.014.4381465
GGTAGTC13250.013.5399867
GCAGCGT14700.013.379821
GTATCCT22400.013.2477821
TCCGCAC9700.013.1595264
GTCCTAG8150.013.1249241
TAGTCAG12650.012.5454329
CAGCGCA11900.012.1764489
CGCACCT9700.012.09253266
GTATAGA24150.011.7162721
GACGCGC6251.8189894E-1211.59175838
CAGCGTC17050.011.536012
GCGCACT9050.011.43633511
CCTCCTA10900.011.3964281
CTAATAC24600.011.3620073
GTATAAT33650.011.2797731
TATTAGG22350.011.2706892
GTGTAGG8350.011.157581
Produced by FastQC (version 0.11.2)