FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996080
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12650.018.5496224
GCAGCGT15600.015.7046661
CAGCGTC15450.015.6344232
TAGGTAG10650.015.2290785
GACGCGC6500.013.79950938
GTATTAG18400.013.6898911
CGCGCAT7000.013.30680440
GTATTAT40500.013.291211
GTATCCT16750.012.7723811
GATGTGT21700.012.08288310
AGCGTCA19800.011.8511493
TAGTCAG10700.011.607539
ACGCGCA7450.011.5767739
CTTAAGG11950.011.2619443
GGTAGTC12800.011.0514947
TGACGCG8200.010.93863737
ACATAGG12350.010.89445931
GTATAAT33000.010.8746261
GACGTAG30350.010.79873669
TCCTAAG11850.010.7744652
Produced by FastQC (version 0.11.2)