FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740415
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG50740.18515443828763162RNA PCR Primer, Index 3 (96% over 28bp)
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATCAAAATCAGCCATTTCCGCACCTTTT32440.11837623133722447No Hit
ATATACATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCG32000.11677063510453708No Hit
CATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCGGTATG28200.1029041221858733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23850.024.8797847
CAGCGTC7150.024.6148622
ATGCCGT24550.024.45142746
CTACGGA5650.024.4245159
GTATGCC24500.024.07889244
CGACCGG5050.023.22704749
GCCGTCT25250.022.543948
ATCACAC11550.022.40194729
CGCACCT11150.022.27737866
CGTCTTC26100.021.54535150
GCGACCG5400.021.0827248
GCGTCAG9300.020.7800351
TGCTTGA27250.020.76269757
CCGTCTT28100.020.74850749
CCTACGG6700.020.597928
CCTAATA15900.020.1845512
CTATGCA13750.020.072519
CGTATGC32200.020.03513143
CCGGCTA5900.019.88077752
TCGTATG31950.019.86796842
Produced by FastQC (version 0.11.2)