FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7598351
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACATGTATACTTAACAGATATGGAATGGTTGGCGAAGTAAATTTTTGGCGACGCGGCGACGCGGCGACGCG86820.11426163387292848No Hit
TCTTACAACTCCGCATACCGCGTCGCCGCGTCGCCGCGTCGCCAAAAATTTACTTCGCCAACCATTCCATATCTG81900.10778654473845707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGT44750.016.65193660
TATGCGG49050.016.10647457
GGTATGC50200.015.94377455
GTATGCG52250.015.25220256
ACTTCGC52350.015.15716752
TATTAGG22100.015.1465932
TGCGGAG50850.015.06142459
CTTCGCC54350.014.78983253
ACTCCGC60900.014.5036778
TAGGTAG15500.014.2492625
CGCCAAC54250.014.18116856
GCGGTAT57500.014.09960453
GTATTAG24350.014.0295311
CGCGGTA59800.014.01883552
ACGCGGT56450.013.93406351
CGGTATG57800.013.90704754
CCATTCC56500.013.73846162
GCCAACC55250.013.7370857
ATGCGGA58850.013.71745158
CCTAATA25350.013.4769852
Produced by FastQC (version 0.11.2)