FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392201
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13850.013.701771
CGTCCGG4406.184564E-1113.32934332
CGCACCT6250.013.24779866
GCGTCAG7500.012.8811334
TAGGTAG7250.012.8497855
TATTAGG13250.012.7598022
GTATAAT23900.012.5598681
CTATACT14650.012.0112964
TATACAG17950.011.9178355
CTAATAC14600.011.3434643
GTATTAT28600.011.2196851
CTAGACC8350.011.1566714
TATACTG22400.011.090595
TAATACT25850.011.0783444
GTATATA34100.011.0289931
CGCGCAT4703.063178E-811.01047140
ACTATAC14500.010.945793
GTATATG20750.010.808341
TATACGG10600.010.7418285
GCAGCGT9450.010.5883891
Produced by FastQC (version 0.11.2)