FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5634091
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12700.017.660535
GTATTAG25450.015.3206461
TAGTCAG13250.012.75820359
GGTAGTC14950.012.69269857
CTCCTAA14200.012.3927651
TAGGGGG4451.36424205E-811.6312224
TATACAG34150.011.6198695
CGCGCAT6550.011.58752540
GACGCGC6550.011.58752538
TATTAGG26000.011.5462252
ACATAGG14750.011.4608631
TCCTAAG15400.011.4272982
CTTAAGG14850.011.3858063
TGCCGTC16000.011.21234747
ATAGGAG16100.011.14270633
GCCGTCT14650.011.06810948
TCCGCAC10150.010.87679664
GTATATA55050.010.8436421
TCTATAC25700.010.7411553
CCTACAC11650.010.6627863
Produced by FastQC (version 0.11.2)