FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499563
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT12100.018.53256266
TCCGCAC11600.018.4390264
CCGCACC13550.015.53095165
TAGGTAG15850.014.5879185
GTATTAG31300.014.2221891
CTAATAC33350.013.6586963
CTCCTAG10400.012.608731
CTCCTAA16800.012.5297271
TATTAGG33550.012.4454492
CGCGCGG4256.9230737E-912.1760554
TAGTCAG15700.012.0864079
CTTACAC18800.011.9312693
CCTACAC10900.011.714033
TAGACTG14500.011.6620775
CCTATAC19550.011.6500633
TCCTTGC35400.011.5983978
TATACTG42400.011.5576715
ACCGTGC7250.011.4216148
GTATAGG23000.011.4026771
TAATACT58950.011.3568814
Produced by FastQC (version 0.11.2)