FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357916
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG9670.2701751248896389TruSeq Adapter, Index 3 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG8590.24000044703226456RNA PCR Primer, Index 3 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG5000.13969758267302942TruSeq Adapter, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCG350.001346763339.42745612
AAGGGGG1600.038.81139869
GGCCCGC451.0003512E-438.3322513
GCCCGCC553.2531228E-431.36274714
ACCGACG500.0077289927.5992188
GCGGTCT700.001332273624.64215913
GAGTACA851.4408676E-424.35225134
AAAGGGG2700.024.2770968
GTATGCC4300.021.66217644
CTGCTTG4350.021.41318756
GCCGTCT4050.021.29569248
TATGCCG4400.021.16985345
TGCCGTC4300.020.85987347
GTCTTAG850.004105424520.3020521
CCCGCCC850.004115404620.29354315
ATGCCGT4650.020.0316946
TCGTATG4500.019.93276842
TGCTTGA4900.019.71372857
CCGTCTT4600.019.49944949
TACACCG900.0057185619.171485
Produced by FastQC (version 0.11.2)