FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104085
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGGA150.00234951669.029
GAATCGC200.00734034151.7568
TATACAG459.953033E-538.3333325
CGTTTTG450.004603594530.66666658
CTATGGT500.00770316227.644
ATGATGC500.00770316227.621
TTGGTTA500.00770316227.657
TATACTG658.604055E-426.5384625
ATATACA851.4310249E-424.3529424
TATATAC1202.4223336E-623.03
GTATATA1356.7257624E-620.4444451
CGAGCCC2651.5796705E-411.7169869
CCGAGCC3004.985447E-410.3568
TCCGAGC3209.023363E-49.70312567
CTCCGAG3250.00104003539.55384666
TCTCCGA3600.0026404588.62565
CATCTCC4653.4721836E-48.1612963
ATCTCCG4000.0068246927.762500364
TACACAT6057.500076E-46.84297569
TATACAC6950.00358469275.956834367
Produced by FastQC (version 0.11.2)