FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919411
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10600.020.8349934
GTATTAG21200.015.7874151
GCAGCGT14500.015.4675131
CAGCGTC15350.014.1624452
CGGTCAG5400.014.05522615
TAGGTAG11950.013.5725815
GGACGGG4901.8189894E-1213.37737450
CCGCACC9350.013.28311265
CCGTGCG2651.1158696E-513.0192249
TCCGCAC9050.012.96102264
TAATACT37250.012.7841574
TATACTG30750.012.4569135
TCGGACG5850.012.38445248
AGCGTCA17650.012.3172893
CGGTCGG4802.6193447E-1012.21846469
CCTCCTA10600.012.0440071
CGCACCT9000.011.88305566
GGTAGTC12000.011.2138337
ACCCGCG4351.2462442E-711.103188533
GTATAGA21800.011.07941151
Produced by FastQC (version 0.11.2)