FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149057
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11650.019.251194
GCAGCGT14300.015.6830521
GTATTAG21150.015.3345391
GTATCCT22500.014.1077771
CAGCGTC15900.014.1054312
TAGGTAG10900.012.9789695
CCGCGCG3601.2893724E-712.4584669
CGCACCT9900.012.19686366
CCGCACC10250.012.11696765
AGCGTCA18600.012.0578693
TCCGCAC10100.011.6137664
CTAATAC25150.011.5242183
TATTAGG19000.011.4408182
CGGTCAG6401.8189894E-1211.32010515
CGCGCAT5906.002665E-1111.10996440
TAGTCAG10750.010.9117459
CGTCAGA20400.010.8251175
GTATAGA22900.010.6973481
TAATACT37850.010.6657194
ATGTGTA25600.010.64659911
Produced by FastQC (version 0.11.2)