FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5079365
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11750.019.3831064
GCAGCGT14250.015.9814621
CAGCGTC14500.015.7063822
CGCACCT9500.014.889029566
GTATTAG25200.014.2403341
CCGCACC10200.014.2054665
TAGGTAG13100.013.6980235
GTATAGA23100.012.995551
CGTCAGA18200.012.8932795
TCCGCAC10700.012.57439164
TAGTCAG12650.012.2730879
AGCGTCA18500.012.1243633
TAATACT41200.011.8934674
TATTAGG24550.011.666132
CTAATAC25650.011.5698683
GGTAGTC12850.011.545887
TATACCG14150.011.4621825
GATGTGT21700.011.44688910
ATGTGTA26800.011.32824311
GTATATA57800.011.2232171
Produced by FastQC (version 0.11.2)