FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679019
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9750.022.6479954
GCAGCGT11100.020.205171
CAGCGTC11450.019.888082
AGCGTCA14000.016.019163
CGTCAGA15450.014.292625
CGCGCAT3901.2732926E-1014.15365140
GACGCGC3153.4579898E-713.14267638
GAGACAG17100.013.11385421
GTATTAG16350.013.0841671
CGCACCT6150.012.34146166
GTATATA38750.012.2879521
GATGTGT18900.012.0477810
GTGTATA20350.012.03685313
GTATAAT24850.011.9410961
TGACGCG3802.6045745E-711.80246837
TAATACT29300.011.7757024
TATACCG10850.011.7660945
ATGTGTA20250.011.755712511
GTCAGAT18900.011.6836486
ACGCGCA4156.490518E-811.63839339
Produced by FastQC (version 0.11.2)