FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447512
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12350.020.1178574
GCAGCGT16050.015.2643941
CAGCGTC16900.014.9052882
GTGCGTG13000.014.065042532
GTATTAG25550.013.7754411
AGCGTCA19050.013.0422843
GGACCGC3255.0410017E-712.7413096
CGTCAGA20700.012.336095
TAGGTAG13500.012.0137975
GCGTACT14900.011.80843852
TATACAG36600.011.5968535
CTAATAC27100.011.4601263
CGCGCGG3054.7158734E-511.311254
GTATATA64100.011.1431651
CGCGGCC3105.565551E-511.12876113
TATTAGG25600.011.0529472
GTATATG34400.011.0339371
GAGACAG22150.010.9026721
TAGGCCG4158.1412145E-710.80706439
TGCGTAC16600.010.80696651
Produced by FastQC (version 0.11.2)