FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401867
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12650.020.185564
TAGGTAG13700.018.6386645
GTATTAG24900.018.29171
CAGCGTC15200.017.252882
GCAGCGT15350.016.6342351
GGTAGTC13850.014.6981157
TAGTCAG14350.014.1849359
AGCGTCA18500.014.1753393
CGCACCT10750.014.12067366
CCGCACC11650.013.32581465
ACATAGG14400.012.69768431
ATAGGAG15500.012.686748533
CTAGACT9050.012.5824434
TATTAGG27150.012.3280242
TCCGCAC11900.011.88622864
TTAGGTA24400.011.7378314
GTATTAT48200.011.4538761
GTATATG32850.011.4490811
GAGACAG23550.011.42654421
TATACGG19250.011.2931085
Produced by FastQC (version 0.11.2)