FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186246
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4310.23141436594611425RNA PCR Primer, Index 33 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG4270.2292666688143638RNA PCR Primer, Index 33 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG2180.1170494936804012RNA PCR Primer, Index 15 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG252.561585E-455.24
GTATTAG602.8930845E-740.251
AAGGGGG1350.038.33333269
GGTGTTC400.002590774634.58
TATACGT553.2466368E-431.3636345
ATATGCC500.007718556627.63
TCTACAC500.007718556627.63
CTACACA500.007718556627.64
CTGTGGG500.007718556627.611
TAATCCT1004.9797745E-727.64
AGTGGGT658.629758E-426.53846228
AAAGGGG2000.025.87568
TACTGGG700.001329655924.6428597
TATAAAG851.4369689E-424.3529432
TATGCCG2150.024.06976943
GTATATG1052.2675238E-523.0000021
TCGTATG2450.022.53061140
GCCGTCT2300.022.50000246
CGTATGC2350.022.02127641
CTCGTAT2350.022.02127641
Produced by FastQC (version 0.11.2)