FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086635
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT7300.020.7940966
CCGCACC8600.018.85426165
TCCGCAC8400.018.48175864
GCGTCAG9000.017.2528084
GCAGCGT10000.016.5618861
GTATTAG18050.016.4395451
TAGGTAG11300.016.1844465
GGTAGTC8950.015.0344967
TAGTCAG9550.013.7283039
CTAATAC20500.013.4654413
CAGCGTC11550.013.4434182
AGCGTCA12300.012.6238523
CGCGGGT3501.23891E-611.82832553
GTATATA49800.011.7091641
GTCTTAG8050.011.5727461
CCTAATA23350.011.526222
TAATACT33900.011.5018724
ACTATGC18800.011.3783988
CTATACT18000.011.3101744
GTATAGA19750.011.181021
Produced by FastQC (version 0.11.2)