FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760584
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2950.022.21919664
CCGCACC3350.018.5363665
GCGTCAG3550.018.4722354
CGCACCT3750.016.55914766
GGTAGTC3209.822543E-1116.1751777
CCTAGCG1756.3624786E-515.77061730
TAGGTAG4300.015.2511975
CTAGACT2756.546361E-815.0606154
CGCGCGG1400.00413341114.78495454
TAGTCAG3101.8158971E-814.467829
CTAATAC8450.014.2957033
CGGGGCT1450.005202412614.2816164
TATTAGG7900.013.9803172
CGCGGGA1500.006532875413.7992956
CTTACAC4557.2759576E-1213.6538533
GTATTAG7700.013.4446851
CCCCTAG1800.001401684713.4197881
CGCTACG1800.001404689913.415977569
CAGCGTC4651.0913936E-1113.3602222
GCAGCGT4457.2759576E-1113.1828411
Produced by FastQC (version 0.11.2)