FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272815
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG6850.019.1425554
GCAGCGT7950.016.9266591
TAGGTAG7000.015.7746195
CAGCGTC9250.014.5486652
GTATTAG14700.014.3181281
TATACTG19400.014.2296815
GGTAGTC7350.013.6131267
GTATAGA12650.013.0925551
GTATATG19950.012.7986021
CCTAGGG2701.3498493E-512.7803613
GTATATA31400.012.7468261
CTCCTAG4651.5279511E-1012.614491
TCTATAC13800.011.7523773
CCCCTAA7050.011.7461591
TCCTTGC19100.011.5609578
ACAGTCC6057.2759576E-1211.4056978
GTATCCT12500.011.3174241
CTATACA15200.011.1239594
TAGTCAG7550.010.9669269
GTCCTAG5703.8926373E-1010.8961081
Produced by FastQC (version 0.11.2)