FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183362
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9250.018.653384
GCAGCGT11450.016.8766351
TCCGCAC7050.016.63794364
GTATTAG17600.015.0966761
CAGCGTC12150.014.20062352
CCGCACC8700.013.87901465
TAGGTAG10000.013.4587355
GTATATG27700.012.9555791
CCTACAC6750.012.7808663
AGCGTCA14100.012.4817393
CGCACCT8600.012.03462866
TATACGG15950.011.8998555
CTAATAC19700.011.5611693
CTAGACT8550.011.3011124
GTATAGA19250.011.29309751
CGCGCGG2752.2698977E-411.29053454
CGTCAGA15600.011.2819785
TATTAGG17750.011.2756942
ACTATGC19150.011.1705428
TAATACT33400.011.0551994
Produced by FastQC (version 0.11.2)